Nicotinic receptors regulate B lymphocyte activation and immune response.
In: European journal of pharmacology, Jg. 517 (2005-07-11), Heft 3, S. 246-51
Online
academicJournal
The presence of nicotinic acetylcholine receptors (nicotinic receptors) composed of either alpha7 or alpha4 and beta2 subunits is revealed in B lymphocytes by means of radioligand binding assay and Cell ELISA. Mouse B lymphocytes contained 12,200+/-3200 of epibatidine-binding sites and 3130+/-750 of alpha-Bungarotoxin-binding sites per cell. Mice lacking nicotinic receptor subunits alpha4, beta2 or alpha7 had less serum IgG and IgG-producing cells in the spleen, but showed stronger immune response to both protein antigen in vivo and CD40-specific antibody in vitro than wild-type mice. Anti-CD40-stimulated proliferation of B lymphocytes from beta2 knockout, but not wild-type mice was inhibited with nicotine. Our results indicate that signalling through nicotinic receptors affects both the pre-immune state and activation of B lymphocytes in the immune response, possibly via CD40-dependent pathway. This could contribute to immune depression found in tobacco smokers.
Titel: |
Nicotinic receptors regulate B lymphocyte activation and immune response.
|
---|---|
Autor/in / Beteiligte Person: | Skok, M ; Grailhe, R ; Changeux, JP |
Link: | |
Zeitschrift: | European journal of pharmacology, Jg. 517 (2005-07-11), Heft 3, S. 246-51 |
Veröffentlichung: | 2005- : Amsterdam : Elsevier Science ; <i>Original Publication</i>: Amsterdam, North Holland Pub. Co., 2005 |
Medientyp: | academicJournal |
ISSN: | 0014-2999 (print) |
DOI: | 10.1016/j.ejphar.2005.05.011 |
Schlagwort: |
|
Sonstiges: |
|