Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins
2021
Online
academicJournal
Zugriff:
Label-free optical detection of biomolecules is currently limited by a lack of specificity rather than sensitivity. To exploit the much more characteristic refractive index dispersion in the mid-infrared (IR) regime, we have engineered three-dimensional IR-resonant silicon micropillar arrays (Si-MPAs) for protein sensing. By exploiting the unique hierarchical nano- and microstructured design of these Si-MPAs attained by CMOS-compatible silicon-based microfabrication processes, we achieved an optimized interrogation of surface protein binding. Based on spatially resolved surface functionalization, we demonstrate controlled three-dimensional interfacing of mammalian cells with Si-MPAs. Spatially controlled surface functionalization for site-specific protein immobilization enabled efficient targeting of soluble and membrane proteins into sensing hotspots directly from cells cultured on Si-MPAs. Protein binding to Si-MPA hotspots at submonolayer level was unambiguously detected by conventional Fourier transform IR spectroscopy. The compatibility with cost-effective CMOS-based microfabrication techniques readily allows integration of this novel IR transducer into fully fledged bioanalytical microdevices for selective and sensitive protein sensing.
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Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins
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Autor/in / Beteiligte Person: | Julia Flesch (10131905) ; Maximilian Bettenhausen (10131908) ; Marcin Kazmierczak (10131911) ; Wolfgang M. Klesse (1866982) ; Oliver Skibitzki (1418923) ; Olympia E. Psathaki (10131914) ; Rainer Kurre (425653) ; Giovanni Capellini (1418896) ; Subhajit Guha (10131917) ; Thomas Schroeder (303739) ; Bernd Witzigmann (7969796) ; Changjiang You (1675261) ; Jacob Piehler (348230) |
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Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
DOI: | 10.1021/acsami.0c22421.s001 |
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