Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins
In: ACS APPLIED MATERIALS & INTERFACES, 2021, Heft 7, S. 8049-8059
Online
academicJournal
Zugriff:
Label-free optical detection of biomolecules is currently limited by a lack of specificity rather than sensitivity. To exploit the much more characteristic refractive index dispersion in the mid-infrared (IR) regime, we have engineered three-dimensional IR-resonant silicon micropillar arrays (Si-MPAs) for protein sensing. By exploiting the unique hierarchical nano- and microstructured design of these Si-MPAs attained by CMOS-compatible silicon-based microfabrication processes, we achieved an optimized interrogation of surface protein binding. Based on spatially resolved surface functionalization, we demonstrate controlled three-dimensional interfacing of mammalian cells with Si-MPAs. Spatially controlled surface functionalization for site-specific protein immobilization enabled efficient targeting of soluble and membrane proteins into sensing hotspots directly from cells cultured on Si-MPAs. Protein binding to Si-MPA hotspots at submonolayer level was unambiguously detected by conventional Fourier transform IR spectroscopy. The compatibility with cost-effective CMOS-based microfabrication techniques readily allows integration of this novel IR transducer into fully fledged bioanalytical microdevices for selective and sensitive protein sensing.
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Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins
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Autor/in / Beteiligte Person: | Flesch, Julia ; Bettenhausen, Maximilian ; Kazmierczak, Marcin ; Klesse, Wolfgang M ; Skibitzki, Oliver ; Psathaki, Olympia E ; Kurre, Rainer ; Capellini, Giovanni ; Guha, Subhajit ; Schroeder, Thomas ; Witzigmann, Bernd ; You, Changjiang ; Piehler, Jacob ; Schroeder, Thoma |
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Zeitschrift: | ACS APPLIED MATERIALS & INTERFACES, 2021, Heft 7, S. 8049-8059 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
DOI: | 10.1021/acsami.0c22421 |
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