The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
In: ISSN: 1538-4101, 2016
Online
academicJournal
Zugriff:
International audience ; MicroRNAs (miRNAs) in the AGO-containing RISC complex control messenger RNA (mRNA) translation by binding to mRNA 3' untranslated region (3'UTR). The relationship between miRNAs and other regulatory factors that also bind to mRNA 3'UTR, such as CPEB1 (cytoplasmic polyadenylation element-binding protein), remains elusive. We found that both CPEB1 and miR-15b control the expression of WEE1, a key mammalian cell cycle regulator. Together, they repress WEE1 protein expression during G1 and S-phase. Interestingly, the 2 factors lose their inhibitory activity at the G2/M transition, at the time of the cell cycle when WEE1 expression is maximal, and, moreover, rather activate WEE1 translation in a synergistic manner. Our data show that translational regulation by RISC and CPEB1 is essential in cell cycle control and, most importantly, is coordinated, and can be switched from inhibition to activation during the cell cycle.
| Titel: |
The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
|
|---|---|
| Autor/in / Beteiligte Person: | Kratassiouk, Gueorgui ; Pritchard, Linda L. ; Cuvellier, Sylvain ; Vislovukh, Andrii ; Meng, Qingwei ; Groisman, Regina ; Degerny, Cindy ; Deforzh, Evgeny ; Harel-Bellan, Annick ; Groisman, Irina ; (PARI), PARi ; Département Plateforme (PF I2BC) ; Institut de Biologie Intégrative de la Cellule (I2BC) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) ; Génétique moléculaire et destin cellulaire (FRE 3377) ; Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) ; Institut Cochin (IC UM3 (UMR 8104 / U1016)) ; Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) ; Department of Translation Mechanisms ; Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine ; The Breast Department ; Third Affiliated Hospital of Harbin Medical University ; Laboratoire Epigénétique et Cancer (LEC) ; Département Biologie des Génomes (DBG) ; Régulation post-transcriptionnelle de l’expression des gènes par les protéines se liant à l’ARN (RPTEG) |
| Link: | |
| Zeitschrift: | ISSN: 1538-4101, 2016 |
| Veröffentlichung: | HAL CCSD ; Taylor & Francis, 2016 |
| Medientyp: | academicJournal |
| DOI: | 10.1080/15384101.2016.1147631 |
| Schlagwort: |
|
| Sonstiges: |
|