Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine
In: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, Jg. 48 (2013), Heft 11-12, S. 1479-1484
academicJournal
- print, 38 ref
Zugriff:
The aim of the study was to determinate the level of expression of silver birch allergen Betvl in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betvl allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Betv1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.
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Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine
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Autor/in / Beteiligte Person: | ZIAROVSKÁ, Jana ; LABAJOVÁ, Mária ; RAZNÁ, Katarína ; BEZO, Milan ; STEFÚNOVÁ, Veronika ; SHEVTSOVA, Tatiana ; GARKAVA, Kateryna ; BRINDZA, Ján |
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Zeitschrift: | Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, Jg. 48 (2013), Heft 11-12, S. 1479-1484 |
Veröffentlichung: | Philadelphia, PA: Taylor & Francis, 2013 |
Medientyp: | academicJournal |
Umfang: | print, 38 ref |
ISSN: | 1093-4529 (print) |
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